Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf].
Identifieur interne : 000D92 ( Main/Exploration ); précédent : 000D91; suivant : 000D93Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf].
Auteurs : Mary-Rus Martínez-Cuenca [Espagne] ; Domingo J. Iglesias ; Manuel Tal N ; Javier Abadía ; Ana-Flor L Pez-Millán ; Eduardo Primo-Millo ; Francisco LegazSource :
- Tree physiology [ 1758-4469 ] ; 2013.
English descriptors
- KwdEn :
- Aconitate Hydratase (genetics), Aconitate Hydratase (metabolism), Carbon (metabolism), Carrier Proteins (genetics), Carrier Proteins (metabolism), Citric Acid (metabolism), Citrus (chemistry), Citrus (enzymology), Citrus (physiology), Cytosol (enzymology), Iron (analysis), Iron (deficiency), Malate Dehydrogenase (genetics), Malate Dehydrogenase (metabolism), Malates (metabolism), Metabolic Networks and Pathways, Mitochondria (enzymology), Phosphoenolpyruvate Carboxylase (genetics), Phosphoenolpyruvate Carboxylase (metabolism), Plant Exudates (chemistry), Plant Leaves (enzymology), Plant Leaves (genetics), Plant Leaves (physiology), Plant Roots (chemistry), Plant Roots (enzymology), Plant Roots (physiology), Poncirus (chemistry), Poncirus (enzymology), Poncirus (physiology), Pyruvate Kinase (genetics), Pyruvate Kinase (metabolism), RNA, Plant (genetics), Seedlings (enzymology), Seedlings (genetics), Seedlings (physiology), Xylem (enzymology), Xylem (genetics), Xylem (physiology).
- MESH :
- chemical , analysis : Iron.
- chemical , chemistry : Plant Exudates.
- chemical , deficiency : Iron.
- chemical , genetics : Aconitate Hydratase, Carrier Proteins, Malate Dehydrogenase, Phosphoenolpyruvate Carboxylase, Pyruvate Kinase, RNA, Plant.
- chemical , metabolism : Aconitate Hydratase, Carbon, Carrier Proteins, Citric Acid, Malate Dehydrogenase, Malates, Phosphoenolpyruvate Carboxylase, Pyruvate Kinase.
- chemistry : Citrus, Plant Roots, Poncirus.
- enzymology : Citrus, Cytosol, Mitochondria, Plant Leaves, Plant Roots, Poncirus, Seedlings, Xylem.
- genetics : Plant Leaves, Seedlings, Xylem.
- physiology : Citrus, Plant Leaves, Plant Roots, Poncirus, Seedlings, Xylem.
- Metabolic Networks and Pathways.
Abstract
The effects of iron (Fe) deficiency on the low-molecular-weight organic acid (LMWOA) metabolism have been investigated in Carrizo citrange (CC) [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.] roots. Major LMWOAs found in roots, xylem sap and root exudates were citrate and malate and their concentrations increased with Fe deficiency. The activities of several enzymes involved in the LMWOA metabolism were also assessed in roots. In the cytosolic fraction, the activities of malate dehydrogenase (cMDH) and phosphoenolpyruvate carboxylase (PEPC) enzymes were 132 and 100% higher in Fe-deficient conditions, whereas the activity of pyruvate kinase was 31% lower and the activity of malic enzyme (ME) did not change. In the mitochondrial fraction, the activities of fumarase, MDH and citrate synthase enzymes were 158, 117 and 53% higher, respectively, in Fe-deficient extracts when compared with Fe-sufficient controls, whereas no significant differences between treatments were found for aconitase (ACO) activity. The expression of their corresponding genes in roots of Fe-deficient plants was higher than that measured in Fe-sufficient controls, except for ACO and ME. Also, dicarboxylate-tricarboxylate carrier (DTC) expression was significantly increased in Fe-deficient roots. In conclusion, Fe deficiency in CC seedlings causes a reprogramming of the carbon metabolism that involves an increase of anaplerotic fixation of carbon via PEPC and MDH activities in the cytosol and a shift of the Krebs cycle in the mitochondria towards a non-cyclic mode, as previously described in herbaceous species. In this scheme, DTC could play an important role shuttling both malate and reducing equivalents between the cytosol and the mitochondria. As a result of this metabolic switch malate and citrate concentrations in roots, xylem sap and root exudates increase.
DOI: 10.1093/treephys/tpt011
PubMed: 23462311
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000488
- to stream PubMed, to step Curation: 000488
- to stream PubMed, to step Checkpoint: 000488
- to stream Ncbi, to step Merge: 001234
- to stream Ncbi, to step Curation: 001234
- to stream Ncbi, to step Checkpoint: 001234
- to stream Main, to step Merge: 000D96
- to stream Main, to step Curation: 000D92
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf].</title><author><name sortKey="Martinez Cuenca, Mary Rus" sort="Martinez Cuenca, Mary Rus" uniqKey="Martinez Cuenca M" first="Mary-Rus" last="Martínez-Cuenca">Mary-Rus Martínez-Cuenca</name><affiliation wicri:level="1"><nlm:affiliation>Department of Citriculture and Vegetal Production, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain. martinez_mru@gva.es</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Citriculture and Vegetal Production, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Iglesias, Domingo J" sort="Iglesias, Domingo J" uniqKey="Iglesias D" first="Domingo J" last="Iglesias">Domingo J. Iglesias</name></author><author><name sortKey="Tal N, Manuel" sort="Tal N, Manuel" uniqKey="Tal N M" first="Manuel" last="Tal N">Manuel Tal N</name></author><author><name sortKey="Abadia, Javier" sort="Abadia, Javier" uniqKey="Abadia J" first="Javier" last="Abadía">Javier Abadía</name></author><author><name sortKey="L Pez Millan, Ana Flor" sort="L Pez Millan, Ana Flor" uniqKey="L Pez Millan A" first="Ana-Flor" last="L Pez-Millán">Ana-Flor L Pez-Millán</name></author><author><name sortKey="Primo Millo, Eduardo" sort="Primo Millo, Eduardo" uniqKey="Primo Millo E" first="Eduardo" last="Primo-Millo">Eduardo Primo-Millo</name></author><author><name sortKey="Legaz, Francisco" sort="Legaz, Francisco" uniqKey="Legaz F" first="Francisco" last="Legaz">Francisco Legaz</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23462311</idno><idno type="pmid">23462311</idno><idno type="doi">10.1093/treephys/tpt011</idno><idno type="wicri:Area/PubMed/Corpus">000488</idno><idno type="wicri:Area/PubMed/Curation">000488</idno><idno type="wicri:Area/PubMed/Checkpoint">000488</idno><idno type="wicri:Area/Ncbi/Merge">001234</idno><idno type="wicri:Area/Ncbi/Curation">001234</idno><idno type="wicri:Area/Ncbi/Checkpoint">001234</idno><idno type="wicri:Area/Main/Merge">000D96</idno><idno type="wicri:Area/Main/Curation">000D92</idno><idno type="wicri:Area/Main/Exploration">000D92</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf].</title><author><name sortKey="Martinez Cuenca, Mary Rus" sort="Martinez Cuenca, Mary Rus" uniqKey="Martinez Cuenca M" first="Mary-Rus" last="Martínez-Cuenca">Mary-Rus Martínez-Cuenca</name><affiliation wicri:level="1"><nlm:affiliation>Department of Citriculture and Vegetal Production, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia, Spain. martinez_mru@gva.es</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Citriculture and Vegetal Production, Instituto Valenciano de Investigaciones Agrarias, 46113 Moncada, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Iglesias, Domingo J" sort="Iglesias, Domingo J" uniqKey="Iglesias D" first="Domingo J" last="Iglesias">Domingo J. Iglesias</name></author><author><name sortKey="Tal N, Manuel" sort="Tal N, Manuel" uniqKey="Tal N M" first="Manuel" last="Tal N">Manuel Tal N</name></author><author><name sortKey="Abadia, Javier" sort="Abadia, Javier" uniqKey="Abadia J" first="Javier" last="Abadía">Javier Abadía</name></author><author><name sortKey="L Pez Millan, Ana Flor" sort="L Pez Millan, Ana Flor" uniqKey="L Pez Millan A" first="Ana-Flor" last="L Pez-Millán">Ana-Flor L Pez-Millán</name></author><author><name sortKey="Primo Millo, Eduardo" sort="Primo Millo, Eduardo" uniqKey="Primo Millo E" first="Eduardo" last="Primo-Millo">Eduardo Primo-Millo</name></author><author><name sortKey="Legaz, Francisco" sort="Legaz, Francisco" uniqKey="Legaz F" first="Francisco" last="Legaz">Francisco Legaz</name></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aconitate Hydratase (genetics)</term><term>Aconitate Hydratase (metabolism)</term><term>Carbon (metabolism)</term><term>Carrier Proteins (genetics)</term><term>Carrier Proteins (metabolism)</term><term>Citric Acid (metabolism)</term><term>Citrus (chemistry)</term><term>Citrus (enzymology)</term><term>Citrus (physiology)</term><term>Cytosol (enzymology)</term><term>Iron (analysis)</term><term>Iron (deficiency)</term><term>Malate Dehydrogenase (genetics)</term><term>Malate Dehydrogenase (metabolism)</term><term>Malates (metabolism)</term><term>Metabolic Networks and Pathways</term><term>Mitochondria (enzymology)</term><term>Phosphoenolpyruvate Carboxylase (genetics)</term><term>Phosphoenolpyruvate Carboxylase (metabolism)</term><term>Plant Exudates (chemistry)</term><term>Plant Leaves (enzymology)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (physiology)</term><term>Plant Roots (chemistry)</term><term>Plant Roots (enzymology)</term><term>Plant Roots (physiology)</term><term>Poncirus (chemistry)</term><term>Poncirus (enzymology)</term><term>Poncirus (physiology)</term><term>Pyruvate Kinase (genetics)</term><term>Pyruvate Kinase (metabolism)</term><term>RNA, Plant (genetics)</term><term>Seedlings (enzymology)</term><term>Seedlings (genetics)</term><term>Seedlings (physiology)</term><term>Xylem (enzymology)</term><term>Xylem (genetics)</term><term>Xylem (physiology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Iron</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Exudates</term></keywords><keywords scheme="MESH" type="chemical" qualifier="deficiency" xml:lang="en"><term>Iron</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Aconitate Hydratase</term><term>Carrier Proteins</term><term>Malate Dehydrogenase</term><term>Phosphoenolpyruvate Carboxylase</term><term>Pyruvate Kinase</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aconitate Hydratase</term><term>Carbon</term><term>Carrier Proteins</term><term>Citric Acid</term><term>Malate Dehydrogenase</term><term>Malates</term><term>Phosphoenolpyruvate Carboxylase</term><term>Pyruvate Kinase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus</term><term>Plant Roots</term><term>Poncirus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term><term>Cytosol</term><term>Mitochondria</term><term>Plant Leaves</term><term>Plant Roots</term><term>Poncirus</term><term>Seedlings</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term><term>Seedlings</term><term>Xylem</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Citrus</term><term>Plant Leaves</term><term>Plant Roots</term><term>Poncirus</term><term>Seedlings</term><term>Xylem</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Metabolic Networks and Pathways</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effects of iron (Fe) deficiency on the low-molecular-weight organic acid (LMWOA) metabolism have been investigated in Carrizo citrange (CC) [Citrus sinensis (L.) Osb. × Poncirus trifoliata (L.) Raf.] roots. Major LMWOAs found in roots, xylem sap and root exudates were citrate and malate and their concentrations increased with Fe deficiency. The activities of several enzymes involved in the LMWOA metabolism were also assessed in roots. In the cytosolic fraction, the activities of malate dehydrogenase (cMDH) and phosphoenolpyruvate carboxylase (PEPC) enzymes were 132 and 100% higher in Fe-deficient conditions, whereas the activity of pyruvate kinase was 31% lower and the activity of malic enzyme (ME) did not change. In the mitochondrial fraction, the activities of fumarase, MDH and citrate synthase enzymes were 158, 117 and 53% higher, respectively, in Fe-deficient extracts when compared with Fe-sufficient controls, whereas no significant differences between treatments were found for aconitase (ACO) activity. The expression of their corresponding genes in roots of Fe-deficient plants was higher than that measured in Fe-sufficient controls, except for ACO and ME. Also, dicarboxylate-tricarboxylate carrier (DTC) expression was significantly increased in Fe-deficient roots. In conclusion, Fe deficiency in CC seedlings causes a reprogramming of the carbon metabolism that involves an increase of anaplerotic fixation of carbon via PEPC and MDH activities in the cytosol and a shift of the Krebs cycle in the mitochondria towards a non-cyclic mode, as previously described in herbaceous species. In this scheme, DTC could play an important role shuttling both malate and reducing equivalents between the cytosol and the mitochondria. As a result of this metabolic switch malate and citrate concentrations in roots, xylem sap and root exudates increase.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Abadia, Javier" sort="Abadia, Javier" uniqKey="Abadia J" first="Javier" last="Abadía">Javier Abadía</name><name sortKey="Iglesias, Domingo J" sort="Iglesias, Domingo J" uniqKey="Iglesias D" first="Domingo J" last="Iglesias">Domingo J. Iglesias</name><name sortKey="L Pez Millan, Ana Flor" sort="L Pez Millan, Ana Flor" uniqKey="L Pez Millan A" first="Ana-Flor" last="L Pez-Millán">Ana-Flor L Pez-Millán</name><name sortKey="Legaz, Francisco" sort="Legaz, Francisco" uniqKey="Legaz F" first="Francisco" last="Legaz">Francisco Legaz</name><name sortKey="Primo Millo, Eduardo" sort="Primo Millo, Eduardo" uniqKey="Primo Millo E" first="Eduardo" last="Primo-Millo">Eduardo Primo-Millo</name><name sortKey="Tal N, Manuel" sort="Tal N, Manuel" uniqKey="Tal N M" first="Manuel" last="Tal N">Manuel Tal N</name></noCountry><country name="Espagne"><noRegion><name sortKey="Martinez Cuenca, Mary Rus" sort="Martinez Cuenca, Mary Rus" uniqKey="Martinez Cuenca M" first="Mary-Rus" last="Martínez-Cuenca">Mary-Rus Martínez-Cuenca</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000D92 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000D92 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23462311
|texte= Metabolic responses to iron deficiency in roots of Carrizo citrange [Citrus sinensis (L.) Osbeck. x Poncirus trifoliata (L.) Raf].
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23462311" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |